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KMID : 0359319920320020181
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Korean Journal of Veterinary Research
1992 Volume.32 No. 2 p.181 ~ p.194
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Relationships between in - vitro virulence - associated characteristics , plasmid - bearing and production of Outer Membrane Protein ( OMP ) of Yersinia enterocolitica isolated from pigs
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Abstract
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Two hundred and eighty nine strains of Yersinia enterocolitica isolated from healthy pigs were tested for the presence of 40∼50 Megadalton virulence-associated plasmids and plasmidmediated in vitro virulence-associated properties, i.e., congo red uptake, calcium dependency, autoagglutination, CRMOX reaction, crystal violet binding and pyrazinamidase reaction. The correlationships between in vitro virulence-associated properties and the presence of 220 Kdalton outer membrane protein(OMP) were examined in strains with or without virulence-associated plasmids. The correlationships between the presence of plasmids on the production of the OMP and the expression of in vitro virulence-associated properties were studied with CRMOX^+ strains and acridine orangecured CRMOX^- mutants. The results were as follows :
1. Of the in vitro virulence-associated tests with 289 strains of Y enterocolitica, 275 strains (95.2%) were positive for pyrazinamidase test, and followed by in order of crystal violet binding test, 226 (79.2% ) : CRMOX test, 190 (65.7%) : autoagglutination test, 1.85(64.0%) : calcium dependency test, 86 (29.8%) and congo red uptake test, 47(16.3%).
2. The correlationship between autoagglutination and CRMOX test(r=0.90) was highly significant (p<0.01).
3. In 190 strains(65.7%) bearing the virulence-associated plasmids(MW 40∼50 Mdalton), the correlation between the presence of plasmids and their in vitro virulence-associated properties were highest with CRMOX test(r=0.93) and followed by in orders of AAG test(0.81), CV test(0.46), PYZ test(0.37) and CD test(0.18), but no correlationship between the presence of plasmids and CR test(-0.11).
4. The CRMOX^+ strains produced the 220 Kdalton OMP when they were cultured at 37℃, but not at 26℃. The presence of 220 Kdalton OMP was correlated significantly with in vitro virulence properties and the presence of virulence-associated plasmid, respectively.
5. In the isogenic CRMOX^- mutant strains, of which plasmid were cured by treatment with acridine orange not only in vitro virulence-associated properties(CR 100% , CD 100% , AAG 82.6% , CV 58.3%) disappeared but also 220 Kdalton OMP(100%) was not produced.
These results indicate that the positive CRMOX reaction is plasmid-mediated and the CRMOX test is potential as an in vitro virulence tests with Y enterocolitica.
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